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验证数据展示

  • WB analysis using 87648-2-RR (same clone as 87648-2-PBS)

    WB analysis using 87648-2-RR (same clone as 87648-2-PBS)

    Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 87648-2-RR (Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87648-2-PBS in a different storage buffer formulation.

  • WB analysis using 87648-2-RR (same clone as 87648-2-PBS)

    WB analysis using 87648-2-RR (same clone as 87648-2-PBS)

    Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87648-2-RR (Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87648-2-PBS in a different storage buffer formulation.

Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) Recombinant monoclonal antibody, PBS Only

Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 87648-2-PBS

产品说明书

CloneNo. 253138E10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

PAK4 (Ser474), PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560), Phospho-PAK4, Phospho-PAK4 (Ser474), Phospho-PAK5 (Ser602)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87648-2-PBS targets Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 p21 protein (Cdc42/Rac)-activated kinase 4
别名 PAK4 (Ser474), PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560), Phospho-PAK4, Phospho-PAK4 (Ser474), Phospho-PAK5 (Ser602)
计算分子量 64 kDa
观测分子量72 kDa
GenBank蛋白编号NM_001014831
基因名称 PAK4
Gene ID (NCBI) 10298
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO96013
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily. Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) refers to the phosphorylated forms of the Group II p21-Activated Kinases (PAKs) at specific serine residues in their kinase activation loops. These phosphorylation events are essential for the catalytic activation of Group II PAKs and serve as definitive markers of their enzymatic activity. (PMID: 17292838)

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