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验证数据展示

  • WB analysis of HeLa using 87394-1-RR

    WB analysis of HeLa using 87394-1-RR

    Non-treated HeLa cells and paclitaxel treated HeLa cells were subjected to SDS PAGE followed by western blot with 87394-1-RR (Phospho-NPM1 (Thr199) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • SEC-HPLC of 87394-1-RR

    SEC-HPLC of 87394-1-RR

    The purity of Human Phospho-NPM1 (Thr199) Recombinant monoclonal antibody (Cat. No. 87394-1-RR) was greater than 95% as determined by SEC-HPLC.

  • FC experiment of Jurkat using 87394-1-RR

    FC experiment of Jurkat using 87394-1-RR

    1x10^6 Jurkat cells untreated (dashed lines) or treated with paclitaxel which intracellularly stained with 0.13 ug Phospho-NPM1 (Thr199) Recombinant monoclonal antibody (87394-1-RR, Clone:252639G7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-NPM1 (Thr199) Recombinant monoclonal antibody

Phospho-NPM1 (Thr199) Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 87394-1-RR

产品说明书

CloneNo. 252639G7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

NPM1 (Thr199), Phospho NPM1 (Thr199), Phospho-NPM, Phospho-NPM (Thr199), Phospho-NPM1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inpaclitaxel treated HeLa cells
Positive FC (Intra) detected inpaclitaxel treated Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy NPM1 Ready-To-Use IHC Kit. NPM1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87394-1-RR targets Phospho-NPM1 (Thr199) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nucleophosmin (nucleolar phosphoprotein B23, numatrin)
别名 NPM1 (Thr199), Phospho NPM1 (Thr199), Phospho-NPM, Phospho-NPM (Thr199), Phospho-NPM1
计算分子量 33 kDa
观测分子量40 kDa
GenBank蛋白编号NM_002520
基因名称 NPM1
Gene ID (NCBI) 4869
RRIDAB_3745512
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP06748
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). Phospho-NPM1 (Thr199) is a phosphorylated form of nucleophosmin (NPM1) at threonine 199, a residue that plays a critical role in cell cycle regulation and cancer development. Indeed, phosphorylation by CDK2/cyclin E on Thr199 residue during G1 phase is critical for the NPM1 dissociation from the centrosome to initiate its duplication and the pre-mRNA processing. (PMID: 26993766)

实验方案

Product Specific Protocols
FC protocol for Phospho-NPM1 (Thr199) antibody 87394-1-RRDownload protocol
WB protocol for Phospho-NPM1 (Thr199) antibody 87394-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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