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验证数据展示

  • WB analysis of HeLa using 87394-1-RR (same clone as 87394-1-PBS)

    WB analysis of HeLa using 87394-1-RR (same clone as 87394-1-PBS)

    Non-treated HeLa cells and paclitaxel treated HeLa cells were subjected to SDS PAGE followed by western blot with 87394-1-RR (Phospho-NPM1 (Thr199) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87394-1-PBS in a different storage buffer formulation.

  • SEC-HPLC of 87394-1-RR

    SEC-HPLC of 87394-1-RR

    The purity of Human Phospho-NPM1 (Thr199) Recombinant monoclonal antibody (Cat. No. 87394-1-RR) was greater than 95% as determined by SEC-HPLC.

  • FC experiment of Jurkat using 87394-1-RR (same clone as 87394-1-PBS)

    FC experiment of Jurkat using 87394-1-RR (same clone as 87394-1-PBS)

    1x10^6 Jurkat cells untreated (dashed lines) or treated with paclitaxel which intracellularly stained with 0.13 ug Phospho-NPM1 (Thr199) Recombinant monoclonal antibody (87394-1-RR, Clone:252639G7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026). This data was developed using the same antibody clone with 87394-1-PBS in a different storage buffer formulation.

Phospho-NPM1 (Thr199) Recombinant monoclonal antibody, PBS Only

Phospho-NPM1 (Thr199) Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 87394-1-PBS

产品说明书

CloneNo. 252639G7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

NPM1 (Thr199), Phospho NPM1 (Thr199), Phospho-NPM, Phospho-NPM (Thr199), Phospho-NPM1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NPM1 Ready-To-Use IHC Kit. NPM1 primary antibody included.

产品信息

87394-1-PBS targets Phospho-NPM1 (Thr199) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nucleophosmin (nucleolar phosphoprotein B23, numatrin)
别名 NPM1 (Thr199), Phospho NPM1 (Thr199), Phospho-NPM, Phospho-NPM (Thr199), Phospho-NPM1
计算分子量 33 kDa
观测分子量40 kDa
GenBank蛋白编号NM_002520
基因名称 NPM1
Gene ID (NCBI) 4869
RRIDAB_3745512
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP06748
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). Phospho-NPM1 (Thr199) is a phosphorylated form of nucleophosmin (NPM1) at threonine 199, a residue that plays a critical role in cell cycle regulation and cancer development. Indeed, phosphorylation by CDK2/cyclin E on Thr199 residue during G1 phase is critical for the NPM1 dissociation from the centrosome to initiate its duplication and the pre-mRNA processing. (PMID: 26993766)

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