验证数据展示
经过测试的应用
| Positive WB detected in | Calyculin A treated HeLa cells, Calyculin A treated NIH/3T3 cells |
| Positive FC (Intra) detected in | Calyculin A treated NIH/3T3 cells |
| Positive ChIP-qPCR detected in | hTNF-α (30 ng/ml, 1 h) HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82335-1-RR targets Phospho-NF-κB p65 (Ser468) in WB, IHC, IF, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, FC (Intra), ELISA, ChIP-qPCR Application Description |
| 文献引用应用 | WB, IHC, IF, ELISA |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, pig, chicken, bovine, sheep, goat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | v-rel reticuloendotheliosis viral oncogene homolog A (avian) |
| 别名 | NFκB (Ser468), NF-κB (Ser468), NF-κB p65 (Ser468), p NF-κB, p NF-κB (Ser468) |
| 计算分子量 | 65 kDa |
| 观测分子量 | 75 kDa |
| GenBank蛋白编号 | BC011603 |
| 基因名称 | NF-κB p65 |
| Gene ID (NCBI) | 5970 |
| RRID | AB_3083091 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q04206 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nuclear factor kB (NF-kB) is a collective term for a small family of dimeric transcription factors [comprising p65 (RelA) and RelB, c-Rel, p50/p105 (NF-κB1), and p52/p100 (NF-κB2)]. All NF-κB proteins share a Rel homology domain (RHD), which is responsible for DNA binding and dimerization. Only p65, RelB, and c-Rel contain potent transactivation domains within sequences from the C-terminal to the RHD. Exterior signals lead to the phosphorylation and degradation of the inhibitory complex IκB, which is modulated by the IκB kinase (IKK), and its degradation allows for the release of the typical NF-κB heterodimer, p65/p50, to translocate into the nucleus. NF-κB binds to its cognate DNA elements and can transcriptionally activate different target genes among which 200-500 genes have been implicated in cell survival/apoptosis, cell growth, immune response, and inflammation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-NF-κB p65 (Ser468) antibody 82335-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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