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Phospho-MYL9 (Thr19/Ser20) Recombinant antibody

Phospho-MYL9 (Thr19/Ser20) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G10

Cat No : 82717-1-RR

Print datasheet

Synonyms

20 kDa myosin light chain, LC20, MLC 2C, MLC2, MRLC1, MYL9, Myosin RLC, MYRL2

验证数据展示

  • WB analysis using 82717-1-RR

    WB analysis using 82717-1-RR

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82717-1-RR (Phospho-MYL9 (Thr19/Ser20) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

经过测试的应用

Positive WB detected inCalyculin A treated NIH/3T3 cells, Calyculin A treated HeLa cells

This antibody, also known as Thr18/Ser19, is the site where the initiator methionine is removed.

Planning an IHC experiment? We recommend our IHCeasy MYL9 Ready-To-Use IHC Kit. MYL9 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82717-1-RR targets Phospho-MYL9 (Thr19/Ser20) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA
Tested Reactivity Human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myosin, light chain 9, regulatory
Synonyms 20 kDa myosin light chain, LC20, MLC 2C, MLC2, MRLC1, MYL9, Myosin RLC, MYRL2
Calculated Molecular Weight 20 kDa
Observed Molecular Weight19-20 kDa
GenBank Accession NumberBC002648
Gene Symbol MYL9
Gene ID (NCBI) 10398
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP24844
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myosin regulatory light polypeptide 9 (MYL9), also known as MLC2, belongs to the myosin regulatory subunits. It plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation at Thr19 and Ser20. Implicated in cytokinesis, receptor capping, and cell locomotion (PMID:11942626, PMID:2526655). Some studies have demonstrated that MYL9 may play important roles in various human cancers. The expression and phosphorylation of MYL9 (Thr19/Ser20) may be increased in human breast (PMID: 22144583) and liver cancers (PMID: 18648664), while decreased in human colon (PMID: 22752057) and bladder cancers (PMID: 21139803). MYL9 was the only gene differentially expressed in the aged versus young injured arteries in the rat smooth muscle cell layers (PMID:22003410).

实验方案

Product Specific Protocols
WB protocol for Phospho-MYL9 (Thr19/Ser20) antibody 82717-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols