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验证数据展示

  • WB analysis using 82717-1-RR

    WB analysis using 82717-1-RR

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82717-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

  • WB analysis using 82717-1-RR

    WB analysis using 82717-1-RR

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82717-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

  • FC experiment of HeLa using 82717-1-RR

    FC experiment of HeLa using 82717-1-RR

    1x10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.25 ug Phospho-Myosin Light Chain 2 (Thr18/Ser19) Recombinant antibody (82717-1-RR, Clone:1G10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-Myosin Light Chain 2 (Thr18/Ser19) Recombinant antibody

Phospho-Myosin Light Chain 2 (Thr18/Ser19) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 82717-1-RR

产品说明书

CloneNo. 1G10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

MYL12A, MYL12B, P MYL12A, P MYL12B, p MYL9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCalyculin A treated NIH/3T3 cells, Calyculin A treated HeLa cells
Positive FC (Intra) detected inCalyculin A treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy MYL9 Ready-To-Use IHC Kit. MYL9 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82717-1-RR targets Phospho-Myosin Light Chain 2 (Thr18/Ser19) in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myosin, light chain 9, regulatory
别名 MYL12A, MYL12B, P MYL12A, P MYL12B, p MYL9
计算分子量 20 kDa
观测分子量19-20 kDa
GenBank蛋白编号BC002648
基因名称 MYL9
Gene ID (NCBI) 10398
RRIDAB_3085363
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP24844
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myosin II is an actin-binding protein composed of MHC (myosin heavy chain), MLCs/MRLCs (myosin regulatory light chains) and ELCs (essential light chains). The Myosin Light Chains are divided into basic MLC (MLC I) and regulatory MLC (MLC II or Myosin Regulatory Light Chain, MRLC). MRLC can be classified into two major types: : class I MRLC found in muscles (MLC1, MLC2, MLC3, MYL4, MYL5, MYL6, MYL7, etc), and class II MRLCs present in non-muscle cells (such as MYL9, MYL12A, MYL12B). Phosphorylation of myosin II regulatory light chain (MRLC) is important for cell motility and cytokinesis in nonmuscle cells. Phosphorylation of MRLC at Ser19 (1P-MRLC) enhances myosin II activity, which is further enhanced by MRLC diphosphorylation at Thr18/Ser19 (2P-MRLC) in vitro .PMID: 23951055,PMID: 21126233
This antibody can recognize the phosphorylation of three highly homologous Myosin-regulated light chains: MYL12A, MYL12B and MYL9.

实验方案

Product Specific Protocols
WB protocol for Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody 82717-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols