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验证数据展示

  • WB analysis of HEK-293 using 80064-2-RR (same clone as 80064-2-PBS)

    WB analysis of HEK-293 using 80064-2-RR (same clone as 80064-2-PBS)

    Non-treated HEK-293 cells and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80064-2-RR (Phospho-MEK6 (Ser207) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80064-2-PBS in a different storage buffer formulation.

  • WB analysis using 80064-2-RR (same clone as 80064-2-PBS)

    WB analysis using 80064-2-RR (same clone as 80064-2-PBS)

    Non-treated NIH/3T3 cells and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80064-2-RR (Phospho-MEK6 (Ser207) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80064-2-PBS in a different storage buffer formulation.

Phospho-MEK6 (Ser207) Recombinant antibody, PBS Only

Phospho-MEK6 (Ser207) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 80064-2-PBS

产品说明书

CloneNo. 250293G8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

MKK6 (Ser207), Phospho-MEK6, Phospho-MEK6(Ser207), Phospho-MKK3 (Ser189), Phospho-MKK3 (Ser189)/MKK6 (Ser207)

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

80064-2-PBS targets Phospho-MEK6 (Ser207) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitogen-activated protein kinase kinase 6
别名 MKK6 (Ser207), Phospho-MEK6, Phospho-MEK6(Ser207), Phospho-MKK3 (Ser189), Phospho-MKK3 (Ser189)/MKK6 (Ser207)
计算分子量 334 aa, 37 kDa
观测分子量38 kDa, 40kDa
GenBank蛋白编号BC012009
基因名称 MEK6
Gene ID (NCBI) 5608
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP52564
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAPKK6 (Dual specificity mitogen activated protein kinase kinase 6), also known as MAP2K6 / MEK6 / MKK6, is a member of MAPKK protein kinase family. MKK6 plays an important role in intracellular signaling pathways leading toward activation of the p38 MAP kinase. MEK6 phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. Phosphorylation of Ser207 and Thr211 by MAP3Ks is critical for MEK6 activation, and acetylation of these resides by VopA blocks MEK6 activity(PMID: 26648936). The antibody also recognizes the ser218 phosphorylation site of MEK3.