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验证数据展示

  • WB analysis using 28903-1-AP

    WB analysis using 28903-1-AP

    Non-treated HEK-293 cells, UV treated HEK-293 cells, non-treated HeLa cells and Anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 28903-1-AP (Phospho-MEK6 (Ser207) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Phospho-MEK6 (Ser207) Polyclonal antibody

Phospho-MEK6 (Ser207) Polyclonal Antibody for WB, ELISA
Cat No. 28903-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

MAP kinase kinase 6, MAP2K6, MAPK/ERK kinase 6, MAPKK 6, MAPKK6, MEK 6, MEK6, MKK6, Phospho-MAP2K6 (Ser207), Phospho-MEK3 (Ser218)/MEK6 (Ser207), Phospho-MEK6 (Ser207), PRKMK6, SAPKK3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inUV treated HEK-293 cells, HEK-293 cells, HeLa cells, Anisomycin treated HeLa cells

The antibody also detects the phosphorylated at Ser218 of the MEK3.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28903-1-AP targets Phospho-MEK6 (Ser207) in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase 6
别名 MAP kinase kinase 6, MAP2K6, MAPK/ERK kinase 6, MAPKK 6, MAPKK6, MEK 6, MEK6, MKK6, Phospho-MAP2K6 (Ser207), Phospho-MEK3 (Ser218)/MEK6 (Ser207), Phospho-MEK6 (Ser207), PRKMK6, SAPKK3
计算分子量 334 aa, 37 kDa
观测分子量35-37 kDa
GenBank蛋白编号BC012009
基因名称 MEK6
Gene ID (NCBI) 5608
RRIDAB_2935411
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52564
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAPKK6 (Dual specificity mitogen activated protein kinase kinase 6), also known as MAP2K6 / MEK6 / MKK6, is a member of MAPKK protein kinase family. MKK6 plays an important role in intracellular signaling pathways leading toward activation of the p38 MAP kinase. MEK6 phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. Phosphorylation of Ser207 and Thr211 by MAP3Ks is critical for MEK6 activation, and acetylation of these resides by VopA blocks MEK6 activity(PMID: 26648936). The antibody also recognizes the ser218 phosphorylation site of MEK3.

实验方案

Product Specific Protocols
WB protocol for Phospho-MEK6 (Ser207) antibody 28903-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols