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验证数据展示

  • WB analysis using 29560-1-AP

    WB analysis using 29560-1-AP

    Non-treated and MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 29560-1-AP (Phospho-MCL1 (Thr163) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as the loading control.

Phospho-MCL1 (Thr163) Polyclonal antibody

Phospho-MCL1 (Thr163) Polyclonal Antibody for WB, ELISA
Cat No. 29560-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMG132 treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy MCL1 Ready-To-Use IHC Kit. MCL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29560-1-AP targets Phospho-MCL1 (Thr163) in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myeloid cell leukemia sequence 1 (BCL2-related)
别名 EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM
计算分子量 350 aa, 37 kDa
观测分子量 40 kDa
GenBank蛋白编号BC017197
基因名称 MCL1
Gene ID (NCBI) 4170
RRIDAB_2935417
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ07820
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MCL1 is an anti-apoptotic member of the BCL-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Recent studies show that MCL1 is upregulated in numerous hematological and solid tumor malignancies. Therefore, MCL1 has been suggested as a potential new therapeutic target. MCL1 can be phosphorylated by several protein kinases which enables the recognition of MCL1 by its E3 ubiquitin-ligases TrCP or FBW7 (PMID: 33308268). MCL1 shows higher stability when phosphorylated on threonine 163 (PMID: 16543145).

实验方案

Product Specific Protocols
WB protocol for Phospho-MCL1 (Thr163) antibody 29560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols