验证数据展示
经过测试的应用
| Positive WB detected in | MG132 treated HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29560-1-AP targets Phospho-MCL1 (Thr163) in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myeloid cell leukemia sequence 1 (BCL2-related) |
| 别名 | EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM |
| 计算分子量 | 350 aa, 37 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC017197 |
| 基因名称 | MCL1 |
| Gene ID (NCBI) | 4170 |
| RRID | AB_2935417 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q07820 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MCL1 is an anti-apoptotic member of the BCL-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Recent studies show that MCL1 is upregulated in numerous hematological and solid tumor malignancies. Therefore, MCL1 has been suggested as a potential new therapeutic target. MCL1 can be phosphorylated by several protein kinases which enables the recognition of MCL1 by its E3 ubiquitin-ligases TrCP or FBW7 (PMID: 33308268). MCL1 shows higher stability when phosphorylated on threonine 163 (PMID: 16543145).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-MCL1 (Thr163) antibody 29560-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
