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验证数据展示

  • WB analysis using 87346-1-RR (same clone as 87346-1-PBS)

    WB analysis using 87346-1-RR (same clone as 87346-1-PBS)

    Non-treated HeLa cells and paclitaxel treated HeLa cells were subjected to SDS PAGE followed by western blot with 87346-1-RR (Phospho-Lamin A/C (Ser22) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87346-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 87346-1-RR (same clone as 87346-1-PBS)

    IHC staining of mouse colon using 87346-1-RR (same clone as 87346-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 87346-1-RR (Phospho-Lamin A/C (Ser22) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87346-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Lamin A/C antibody included
  • IHC staining of mouse colon using 87346-1-RR (same clone as 87346-1-PBS)

    IHC staining of mouse colon using 87346-1-RR (same clone as 87346-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 87346-1-RR (Phospho-Lamin A/C (Ser22) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87346-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Lamin A/C antibody included

Phospho-Lamin A/C (Ser22) Recombinant monoclonal antibody, PBS Only

Phospho-Lamin A/C (Ser22) Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 87346-1-PBS

产品说明书

CloneNo. 252663H8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

Phospho-Lamin A/C, Phospho-lamin-A, Phospho-lamin-C, p-lamin A/C, p-Lamin A/C (S22)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Lamin A/C Ready-To-Use IHC Kit. Lamin A/C primary antibody included.

产品信息

87346-1-PBS targets Phospho-Lamin A/C (Ser22) in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lamin A/C
别名 Phospho-Lamin A/C, Phospho-lamin-A, Phospho-lamin-C, p-lamin A/C, p-Lamin A/C (S22)
计算分子量 65 kDa
观测分子量 65 kDa, 70 kDa
GenBank蛋白编号NM_170707
基因名称 Lamin A/C
Gene ID (NCBI) 4000
ENSEMBL Gene IDENSG00000160789
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP02545
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Lamin A/C is also named as LMNA or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. Phospho-Lamin A/C (Ser22) is a phosphorylated form of Lamin A/C, which is a protein that plays a crucial role in the structure and function of the nuclear lamina. Lamin A/C phosphorylation is predicted to have regulatory consequences.

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