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验证数据展示

  • WB analysis using 87309-1-RR

    WB analysis using 87309-1-RR

    Non-treated NIH/3T3 cells and etoposide treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87309-1-RR (Phospho-KAP1 (Ser824) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • IF Staining of NIH/3T3 using 87309-1-RR

    IF Staining of NIH/3T3 using 87309-1-RR

    Immunofluorescent analysis of (4% PFA) fixed etoposide treated NIH/3T3 cells using Phospho-KAP1 (Ser824) antibody (87309-1-RR, Clone: 252561H1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of NIH/3T3 using 87309-1-RR

    IF Staining of NIH/3T3 using 87309-1-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Phospho-KAP1 (Ser824) antibody (87309-1-RR, Clone: 252561H1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Phospho-KAP1 (Ser824) Recombinant monoclonal antibody

Phospho-KAP1 (Ser824) Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87309-1-RR

产品说明书

CloneNo. 252561H1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Phospho KAP1 (Ser824), Phospho-KAP1, Phospho-KAP-1, Phospho-KAP-1 (Ser824), Phospho-KAP1(Ser824)

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inetoposide treated NIH/3T3 cells
Positive IF/ICC detected inNIH/3T3 cells, etoposide treated NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy KAP1 Ready-To-Use IHC Kit. KAP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87309-1-RR targets Phospho-KAP1 (Ser824) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tripartite motif-containing 28
别名 Phospho KAP1 (Ser824), Phospho-KAP1, Phospho-KAP-1, Phospho-KAP-1 (Ser824), Phospho-KAP1(Ser824)
计算分子量 89 kDa
观测分子量110 kDa
GenBank蛋白编号NM_005762
基因名称 KAP1
Gene ID (NCBI) 10155
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13263
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. Phospho-KAP1 (Ser824) refers to the phosphorylated form of KAP1 (KRAB-associated protein 1) at Serine 824. This post-translational modification plays a significant role in the regulation of gene expression and chromatin structure.

实验方案

Product Specific Protocols
IF protocol for Phospho-KAP1 (Ser824) antibody 87309-1-RRDownload protocol
WB protocol for Phospho-KAP1 (Ser824) antibody 87309-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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