验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, NIH/3T3 cells, HSC-T6 cells, UV treated HEK-293 cells, UV treated NIH/3T3 cells, UV treated HSC-T6 cells, Anisomycin treated HeLa cells |
| Positive IHC detected in | Jurkat cells Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 28 publications below |
| IHC | See 3 publications below |
产品信息
60666-1-Ig targets Phospho-JNK (Thr183/Tyr185) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, chicken |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase 8 |
| 别名 | JNK (Thr183/Tyr185), Phospho-JNK, phospho-JNK(Thr183/Tyr185), phospho‐JNK/SAPK (Thr183/Tyr185), phospho-JNK1/2 (Thr183/Tyr185) |
| 计算分子量 | 48 kDa |
| 观测分子量 | 42 kDa, 50 kDa |
| GenBank蛋白编号 | NM_138982 |
| 基因名称 | JNK |
| Gene ID (NCBI) | 5599 |
| RRID | AB_3743229 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P45983 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. The antibody can detect endogenous levels of p46 and p54 SAPK/JNK when phosphorylated at Thr183 and Tyr185. It will also react with JNK singly phosphorylated at Thr183.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Phospho-JNK (Thr183/Tyr185) antibody 60666-1-Ig | Download protocol |
| WB protocol for Phospho-JNK (Thr183/Tyr185) antibody 60666-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Activating the Osteoblastic USP26 Pathway Alleviates Multi-Organ Fibrosis by Decreasing Insulin Resistance. | ||
Cancer Lett Histone lactylation-derived LINC01127 promotes the self-renewal of glioblastoma stem cells via the cis-regulating the MAP4K4 to activate JNK pathway. | ||
Mater Today Bio Proanthocyanidin capsules remodel the ROS microenvironment via regulating MAPK signaling for accelerating diabetic wound healing | ||
Adv Healthc Mater Source-Dependent Mitochondrial Transplantation Drives Schwann Cell Bioenergetic Reprogramming and Peripheral Nerve Regeneration. | ||
J Transl Med Nicotine promotes cardiac fibrosis by regulating miR-125b-5p maturation via HNRNPA2B1-mediated METTL14-dependent m⁶A methylation: therapeutic reversal by cinacalcet HCl. | ||
Int J Biol Macromol Isolation, structural characterization and immunoregulatory activity of a novel acidic polysaccharide from Ganoderma sinense. |
