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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 85238-1-RR (same clone as 85238-1-PBS)

    WB analysis using 85238-1-RR (same clone as 85238-1-PBS)

    Non-treated NIH/3T3 cells and TNF alpha treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85238-1-RR (Phospho-IRS1 (Ser307) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85238-1-PBS in a different storage buffer formulation.

  • WB analysis using 85238-1-RR (same clone as 85238-1-PBS)

    WB analysis using 85238-1-RR (same clone as 85238-1-PBS)

    Non-treated and TNF alpha treated HeLa cells were subjected to SDS PAGE followed by western blot with 85238-1-RR (Phospho-IRS1 (Ser307) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85238-1-PBS in a different storage buffer formulation.

Phospho-IRS1 (Ser307) Recombinant antibody, PBS Only

Phospho-IRS1 (Ser307) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85238-1-PBS

产品说明书

CloneNo. 242124E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

IRS1 (Ser307), P IRS1 (Ser307), Phospho IRS1, Phospho IRS1 (Ser307), Phospho-IRS1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85238-1-PBS targets Phospho-IRS1 (Ser307) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 insulin receptor substrate 1
别名 IRS1 (Ser307), P IRS1 (Ser307), Phospho IRS1, Phospho IRS1 (Ser307), Phospho-IRS1
计算分子量 1242 aa, 132 kDa
观测分子量180 kDa
GenBank蛋白编号BC053895
基因名称 IRS1
Gene ID (NCBI) 3667
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35568
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Serine phosphorylation of insulin receptor substrate-1 (IRS-1) inhibits insulin signal transduction in a variety of cell backgrounds, which might contribute to peripheral insulin resistance. However, because of the large number of potential phosphorylation sites, the mechanism of inhibition has been difficult to determine. It has been confirmed that JNK and IKK are involved in the phosphorylation of IRS-1 (Ser307/312). Additionally, activation of Akt, ERK, mTOR, and PKCζ correlates to IRS-1 phosphorylation at Ser307/312, Ser267/270, and Ser612/616 in rodent/human IRS-1. (PMID: 11606564, PMID: 12714600)