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验证数据展示

  • WB analysis using 85238-1-RR

    WB analysis using 85238-1-RR

    Non-treated NIH/3T3 cells and TNF alpha treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85238-1-RR (Phospho-IRS1 (Ser307) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

  • WB analysis using 85238-1-RR

    WB analysis using 85238-1-RR

    Non-treated and TNF alpha treated HeLa cells were subjected to SDS PAGE followed by western blot with 85238-1-RR (Phospho-IRS1 (Ser307) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

Phospho-IRS1 (Ser307) Recombinant antibody

Phospho-IRS1 (Ser307) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85238-1-RR

产品说明书

CloneNo. 242124E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

IRS1 (Ser307), P IRS1 (Ser307), Phospho IRS1, Phospho IRS1 (Ser307), Phospho-IRS1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, TNF alpha treated HeLa cells, TNF alpha treated NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85238-1-RR targets Phospho-IRS1 (Ser307) in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 insulin receptor substrate 1
别名 IRS1 (Ser307), P IRS1 (Ser307), Phospho IRS1, Phospho IRS1 (Ser307), Phospho-IRS1
计算分子量 1242 aa, 132 kDa
观测分子量180 kDa
GenBank蛋白编号BC053895
基因名称 IRS1
Gene ID (NCBI) 3667
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35568
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serine phosphorylation of insulin receptor substrate-1 (IRS-1) inhibits insulin signal transduction in a variety of cell backgrounds, which might contribute to peripheral insulin resistance. However, because of the large number of potential phosphorylation sites, the mechanism of inhibition has been difficult to determine. It has been confirmed that JNK and IKK are involved in the phosphorylation of IRS-1 (Ser307/312). Additionally, activation of Akt, ERK, mTOR, and PKCζ correlates to IRS-1 phosphorylation at Ser307/312, Ser267/270, and Ser612/616 in rodent/human IRS-1. (PMID: 11606564, PMID: 12714600)

实验方案

Product Specific Protocols
WB protocol for Phospho-IRS1 (Ser307) antibody 85238-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols