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验证数据展示

  • WB analysis of Karpas-299 using 85753-1-RR (same clone as 85753-1-PBS)

    WB analysis of Karpas-299 using 85753-1-RR (same clone as 85753-1-PBS)

    Non-treated Karpas-299 cells and IL-1 beta treated Karpas-299 cells were subjected to SDS PAGE followed by western blot with 85753-1-RR (Phospho-IRAK4 (Thr345/Ser346) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85753-1-PBS in a different storage buffer formulation.

  • IHC staining of rat lymph node using 85753-1-RR (same clone as 85753-1-PBS)

    IHC staining of rat lymph node using 85753-1-RR (same clone as 85753-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lymph node tissue slide using 85753-1-RR (Phospho-IRAK4 (Thr345/Ser346) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85753-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IRAK4 antibody included

Phospho-IRAK4 (Thr345/Ser346) Recombinant monoclonal antibody, PBS Only

Phospho-IRAK4 (Thr345/Ser346) Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85753-1-PBS

产品说明书

CloneNo. 242271A8

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, Indirect ELISA

别名

IRAK4 (Ser346), IRAK4 (Thr345), IRAK4 (Thr345/Ser346), P IRAK4 (Thr345/Ser346), Phospho IRAK4 (Thr345/Ser346)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® IRAK4 Ready-To-Use IHC Kit. IRAK4 primary antibody included.

产品信息

85753-1-PBS targets Phospho-IRAK4 (Thr345/Ser346) in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin-1 receptor-associated kinase 4
别名 IRAK4 (Ser346), IRAK4 (Thr345), IRAK4 (Thr345/Ser346), P IRAK4 (Thr345/Ser346), Phospho IRAK4 (Thr345/Ser346)
计算分子量 52 kDa
观测分子量55 kDa
GenBank蛋白编号BC013316
基因名称 IRAK4
Gene ID (NCBI) 51135
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NWZ3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IRAK4 is the central kinase in the Toll-like receptor pathway of the innate immune system. Toll-like receptors constitute the first line of defense against pathogenic micro-organisms such as bacteria, viruses, and yeast. Unphosphorylated full-length IRAK4 can autophosphorylate in vitro in a cell-free system. In the cellular context, however, autophosphorylation is inducible by IL-1β. Four phosphorylation sites have been identified in the activation loop in some articles, which are the results of autophosphorylation: Thr-342, Thr-345, Ser-346 and Thr-352. (PMID: 24567333)

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