验证数据展示
经过测试的应用
| Positive WB detected in | IL-1 beta treated Karpas-299 cells |
| Positive IHC detected in | rat lymph node tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85753-1-RR targets Phospho-IRAK4 (Thr345/Ser346) in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | interleukin-1 receptor-associated kinase 4 |
| 别名 | IRAK4 (Ser346), IRAK4 (Thr345), IRAK4 (Thr345/Ser346), P IRAK4 (Thr345/Ser346), Phospho IRAK4 (Thr345/Ser346) |
| 计算分子量 | 52 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC013316 |
| 基因名称 | IRAK4 |
| Gene ID (NCBI) | 51135 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9NWZ3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IRAK4 is the central kinase in the Toll-like receptor pathway of the innate immune system. Toll-like receptors constitute the first line of defense against pathogenic micro-organisms such as bacteria, viruses, and yeast. Unphosphorylated full-length IRAK4 can autophosphorylate in vitro in a cell-free system. In the cellular context, however, autophosphorylation is inducible by IL-1β. Four phosphorylation sites have been identified in the activation loop in some articles, which are the results of autophosphorylation: Thr-342, Thr-345, Ser-346 and Thr-352. (PMID: 24567333)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Phospho-IRAK4 (Thr345/Ser346) antibody 85753-1-RR | Download protocol |
| WB protocol for Phospho-IRAK4 (Thr345/Ser346) antibody 85753-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
