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验证数据展示

  • WB analysis of HeLa using 84739-2-RR

    WB analysis of HeLa using 84739-2-RR

    Calyculin A treated and untreated HeLa cells were subjected to SDS PAGE followed by western blot with 84739-2-RR (Phospho-Histone H3 (Thr3) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • FC experiment of NIH/3T3 using 84739-2-RR

    FC experiment of NIH/3T3 using 84739-2-RR

    1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-Histone H3 (Thr3) Recombinant antibody (84739-2-RR, Clone:241897D9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-Histone H3 (Thr3) Recombinant monoclonal antibody

Phospho-Histone H3 (Thr3) Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84739-2-RR

产品说明书

CloneNo. 241897D9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

Histone H3 (Thr3), p Histone H3, p Histone H3 (Thr3), p Histone H3 Thr3, p-Histone H3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCalyculin A treated HeLa cells
Positive FC (Intra) detected inCalyculin A treated NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84739-2-RR targets Phospho-Histone H3 (Thr3) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H3a
别名 Histone H3 (Thr3), p Histone H3, p Histone H3 (Thr3), p Histone H3 Thr3, p-Histone H3
观测分子量17 kDa
GenBank蛋白编号BC066245
基因名称 HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_3672124
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP68431
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Phospho-Histone H3 (Thr3) antibody 84739-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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