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验证数据展示

  • WB analysis using 84739-1-RR (same clone as 84739-1-PBS)

    WB analysis using 84739-1-RR (same clone as 84739-1-PBS)

    Non-treated HeLa cels, NIH/3T3 cells, HSC-T6 cells, and Calyculin A treated HeLa cels, NIH/3T3 cells, HSC-T6 cells were subjected to SDS PAGE followed by western blot with 84739-1-RR (Phospho-Histone H3 (Thr3) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84739-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84739-1-RR (same clone as 84739-1-PBS)

    IF Staining of HeLa using 84739-1-RR (same clone as 84739-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84739-1-RR, Clone: 241897G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84739-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84739-1-RR (same clone as 84739-1-PBS)

    FC experiment of HeLa using 84739-1-RR (same clone as 84739-1-PBS)

    1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-Histone H3 (Thr3) Recombinant antibody (84739-1-RR, Clone:241897G3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 84739-1-PBS in a different storage buffer formulation.

Phospho-Histone H3 (Thr3) Recombinant antibody, PBS Only

Phospho-Histone H3 (Thr3) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84739-1-PBS

产品说明书

CloneNo. 241897G3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

HIST1H3A, 241897G3, Histone-H3, HistoneH3, Histone H3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

产品信息

84739-1-PBS targets Phospho-Histone H3 (Thr3) in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H3a
别名 HIST1H3A, 241897G3, Histone-H3, HistoneH3, Histone H3
观测分子量15 kDa
GenBank蛋白编号BC066245
基因名称 HIST1H3A
Gene ID (NCBI) 8350
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP68431
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Phospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. On the basis of previous research, a few cell line- and animal model-based researches have displayed an increase in phosphorylation of histone H3 at Ser10 (H3S10ph), the only histone marker that is involved in carcinogenesis and cellular transformation. Histone H3 phosphorylation on serine-10 is specific to mitosis and phosphorylated histone H3 (PHH3) proliferation markers (as counts defined per area or as indices defined per cell numbers) are increasingly being used to evaluate proliferation in various tumors.