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验证数据展示

  • WB analysis using 82762-11-RR

    WB analysis using 82762-11-RR

    Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 82762-11-RR (Phospho-HER2/ErbB2 (Tyr877) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • IF Staining of A431 using 82762-11-RR

    IF Staining of A431 using 82762-11-RR

    Immunofluorescent analysis of (4% PFA) fixed EGF treated A431 cells using Phospho-HER2/ErbB2 (Tyr877) antibody (82762-11-RR, Clone: 250539F5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of A431 using 82762-11-RR

    FC experiment of A431 using 82762-11-RR

    1X10^6 A431 cells untreated (dashed lines) or treated with EGF which intracellularly stained with 0.25 ug Phospho-HER2/ErbB2 (Tyr877) Recombinant monoclonal antibody (82762-11-RR, Clone:250539F5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-HER2/ErbB2 (Tyr877) Recombinant monoclonal antibody

Phospho-HER2/ErbB2 (Tyr877) Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 82762-11-RR

产品说明书

CloneNo. 250539F5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

ErbB2 (Tyr877), HER2 (Tyr877), HER2/ErbB2 (Tyr877), P-HER2/ErbB2, P-HER2/ErbB2 (Tyr877)

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inEGF treated A431 cells
Positive IF/ICC detected inEGF treated A431 cells
Positive FC (Intra) detected inEGF treated A431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82762-11-RR targets Phospho-HER2/ErbB2 (Tyr877) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
别名 ErbB2 (Tyr877), HER2 (Tyr877), HER2/ErbB2 (Tyr877), P-HER2/ErbB2, P-HER2/ErbB2 (Tyr877)
计算分子量 1255 aa, 138 kDa
观测分子量185 kDa
GenBank蛋白编号BC156755
基因名称 HER2/ErbB2
Gene ID (NCBI) 2064
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP04626
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. Phospho-HER2/ErbB2 (Tyr877) refers to the phosphorylated form of the human epidermal growth-factor receptor 2 (HER2, ErbB2) at tyrosine 877 within its kinase domain. This phosphorylation event is catalyzed in trans by dimerization-activated HER2 itself or by Src-family kinases (SFKs) upon receptor clustering, and serves as a critical activation switch for downstream signaling cascades. ( PMID: 34107301)

实验方案

Product Specific Protocols
FC protocol for Phospho-HER2/ErbB2 (Tyr877) antibody 82762-11-RRDownload protocol
IF protocol for Phospho-HER2/ErbB2 (Tyr877) antibody 82762-11-RRDownload protocol
WB protocol for Phospho-HER2/ErbB2 (Tyr877) antibody 82762-11-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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