验证数据展示
经过测试的应用
| Positive WB detected in | human Neuregulin-1 treated T-47D cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87096-1-RR targets Phospho-HER2/ErbB2 (Tyr1248) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) |
| 别名 | ErbB2 (phospho-Y1248), HER2 (phospho-Y1248), p-ErbB2 Y1248, p-HER2 Y1248, Phospho-ErbB2 |
| 计算分子量 | 1255 aa, 138 kDa |
| 观测分子量 | 185 kDa |
| GenBank蛋白编号 | BC156755 |
| 基因名称 | HER2/ErbB2 |
| Gene ID (NCBI) | 2064 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04626 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. Phospho-HER2/ErbB2 (Tyr1248) is a phosphorylated form of the HER2 protein, which is a member of the epidermal growth factor receptor (EGFR) family. This phosphorylation event is considered a prerequisite for HER2 signaling and its transforming activity. The phosphorylation of HER2 at Tyr1248 residue is a key step in the activation of downstream signaling cascades that regulate various cellular processes, including cell growth, proliferation, and survival. Tyrosine 1248 is the main autophosphorylation site in the carboxy terminal domain of ErbB2 linked to downstream signaling through the ras-raf-MEK-p42/44 MAPK signal transduction pathway. (PMID: 19084911)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-HER2/ErbB2 (Tyr1248) antibody 87096-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
