验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, Cobalt Chloride treated MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy FOXO1 Ready-To-Use IHC Kit. FOXO1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
28757-1-AP targets Phospho-FOXO1 (Ser319) in WB, IF, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human |
| 文献引用反应性 | human, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | forkhead box O1 |
| 别名 | FKH1, FKHR, forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1, FOXO1A, Phospho-FOXO1 (Ser319) |
| 计算分子量 | 655 aa, 70 kDa |
| 观测分子量 | 70-80 kDa |
| GenBank蛋白编号 | BC021981 |
| 基因名称 | FOXO1 |
| Gene ID (NCBI) | 2308 |
| RRID | AB_3669669 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q12778 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The forkhead box class O (FoxO) family in mammals is comprised of the evolutionally highly conserved forkhead transcription factors, FoxO1, FoxO3a, FoxO4, and FoxO6. FoxO1 is highly expressed in lymphoid cells, especially in T and B cells. FoxO1 phosphorylation determines the transcriptional activity and cellular localization of FoxO1. Results showed that GLK-IKKβ signaling triggered the nuclear export of FoxO1. (PMID: 35966593)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-FOXO1 (Ser319) antibody 28757-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Parasit Vectors The effect of Toxoplasma gondii infection on galectin-9 expression in decidual macrophages contributing to dysfunction of decidual NK cells during pregnancy | ||
J Ethnopharmacol HouShiHeiSan attenuates sarcopenia in Middle Cerebral Artery Occlusion (MCAO) rats |