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验证数据展示

  • WB analysis using 84906-1-RR (same clone as 84906-1-PBS)

    WB analysis using 84906-1-RR (same clone as 84906-1-PBS)

    Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 84906-1-RR (Phospho-EGFR (Tyr1197) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84906-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 84906-1-RR (same clone as 84906-1-PBS)

    FC experiment of A431 using 84906-1-RR (same clone as 84906-1-PBS)

    1x10^6 A431 cells untreated (dashed lines) or treated with EGF which intracellularly stained with 0.25 ug Phospho-EGFR (Tyr1197) Recombinant Antibody (84906-1-RR, Clone:241826G10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 84906-1-PBS in a different storage buffer formulation.

Phospho-EGFR (Tyr1197) Recombinant monoclonal antibody, PBS Only

Phospho-EGFR (Tyr1197) Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84906-1-PBS

产品说明书

CloneNo. 241826G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

EGF Receptor (Tyr1173), EGFR (Tyr1197), p-EGF Receptor, p-EGF Receptor (Tyr1173), p-EGFR

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EGFR Ready-To-Use IHC Kit. EGFR primary antibody included.

产品信息

84906-1-PBS targets Phospho-EGFR (Tyr1197) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
别名 EGF Receptor (Tyr1173), EGFR (Tyr1197), p-EGF Receptor, p-EGF Receptor (Tyr1173), p-EGFR
计算分子量 1210 aa, 134 kDa
观测分子量175 kDa
GenBank蛋白编号BC094761
基因名称 EGFR
Gene ID (NCBI) 1956
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP00533
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). The EGFR autophosphorylation sites Tyr-992 and Tyr-1173 (also known as Tyr 1197) have been shown to play a critical role in the activation of the MAPK cascade following EGF stimulation. (PMID: 15708852) Some scientists call EGFR Tyr1197 as EGFR Tyr1173, the site where the signaling peptide is removed.

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