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验证数据展示

  • WB analysis using 87084-2-RR

    WB analysis using 87084-2-RR

    Non-treated A431 cells and EGF treated A431 cells were subjected to SDS PAGE followed by western blot with 87084-2-RR (Phospho-EGFR (Tyr1110) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

  • Schematic diagram of the phosphorylation sites of EGFR.

    Schematic diagram of the phosphorylation sites of EGFR.

    In the full-length versus signal-peptide-removed forms, nomenclature differs but the modification site is conserved.

  • IF Staining of A431 using 87084-2-RR

    IF Staining of A431 using 87084-2-RR

    Immunofluorescent analysis of (4% PFA) fixed EGF treated A431 cells using Phospho-EGFR (Tyr1110) antibody (87084-2-RR, Clone: 251800D1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of A431 using 87084-2-RR

    FC experiment of A431 using 87084-2-RR

    1X10^6 A431 cells untreated (dashed lines) or treated with EGF which intracellularly stained with 0.13 ug Phospho-EGFR (Tyr1110) Recombinant monoclonal antibody (87084-2-RR, Clone:251800D1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-EGFR (Tyr1110) Recombinant monoclonal antibody

Phospho-EGFR (Tyr1110) Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 87084-2-RR

产品说明书

CloneNo. 251800D1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

p-EGF Receptor, p-EGF Receptor (Tyr1086), p-EGFR (Tyr1086), p-EGFR (Tyr1110), p-EGFR Y1086

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inEGF treated A431 cells
Positive IF/ICC detected inEGF treated A431 cells
Positive FC (Intra) detected inEGF treated100ng/ml ,30min A431 cells

This antibody, also known as EGFR Tyr1086, is the site where the signal peptide is removed.

Planning an IHC experiment? We recommend our IHCeasy EGFR Ready-To-Use IHC Kit. EGFR primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87084-2-RR targets Phospho-EGFR (Tyr1110) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
别名 p-EGF Receptor, p-EGF Receptor (Tyr1086), p-EGFR (Tyr1086), p-EGFR (Tyr1110), p-EGFR Y1086
计算分子量 1210 aa, 134 kDa
观测分子量175 kDa
GenBank蛋白编号BC094761
基因名称 EGFR
Gene ID (NCBI) 1956
RRIDAB_3745324
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP00533
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EGFR is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Phospho-EGFR (Tyr1110) is the phosphorylated, active form of the epidermal growth factor receptor (EGFR) at tyrosine 1110 within the C-terminal tail. This modification is catalyzed by EGFR itself or SRC-family kinases upon ligand (EGF, TGF-α) binding or oxidative stress. Phosphorylation at Tyr1110 creates a docking site for SH2-domain-containing proteins, propagating downstream signals through the RAS-RAF-MEK-ERK and PI3K-AKT pathways. Some scientists call EGFR Tyr1110 as EGFR Tyr1086, the site where the signaling peptide is removed.

实验方案

Product Specific Protocols
FC protocol for Phospho-EGFR (Tyr1110) antibody 87084-2-RRDownload protocol
IF protocol for Phospho-EGFR (Tyr1110) antibody 87084-2-RRDownload protocol
WB protocol for Phospho-EGFR (Tyr1110) antibody 87084-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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