验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | EGF treated A431 cells |
This antibody, also known as EGFR Tyr992, is the site where the signal peptide is removed.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-82736-11 targets Phospho-EGFR (Tyr1016) in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) |
| 别名 | EGF Receptor (Tyr992), EGFR (Tyr1016), EGFR (Tyr992), p EGFR (Tyr1016), p-EGF Receptor (Tyr992) |
| 计算分子量 | 1210 aa, 134 kDa |
| 观测分子量 | 170 kDa |
| GenBank蛋白编号 | BC094761 |
| 基因名称 | EGFR |
| Gene ID (NCBI) | 1956 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 激发激光 | Red Laser (633 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P00533 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). Phosphorylation at Tyr1016 is essential for the recruitment of downstream signaling molecules. For example, it can interact with adapter proteins like GRB2, which in turn activates the Ras-MAPK pathway, leading to cell proliferation and differentiation. (PMID: 26628682) Some scientists call EGFR Tyr1016 as EGFR Tyr992, the site where the signaling peptide is removed.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 Phospho-EGFR (Tyr1016) antibody CL647-82736-11 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
