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验证数据展示

  • WB analysis using 85246-1-RR (same clone as 85246-1-PBS)

    WB analysis using 85246-1-RR (same clone as 85246-1-PBS)

    Non-treated NIH/3T3 cells and MG132 treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85246-1-RR (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85246-1-PBS in a different storage buffer formulation.

  • WB analysis of HT-1080 using 85246-1-RR (same clone as 85246-1-PBS)

    WB analysis of HT-1080 using 85246-1-RR (same clone as 85246-1-PBS)

    Non-treated HT-1080 cells and MG132 treated HT-1080 cells were subjected to SDS PAGE followed by western blot with 85246-1-RR (Phospho-Cyclin D1 (Thr286) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85246-1-PBS in a different storage buffer formulation.

Phospho-Cyclin D1 (Thr286) Recombinant monoclonal antibody, PBS Only

Phospho-Cyclin D1 (Thr286) Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85246-1-PBS

产品说明书

CloneNo. 242211A1

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, Indirect ELISA

别名

Cyclin D1 (Thr286), p Cyclin D1, p Cyclin D1 (Thr286), p-Cyclin D1, p-Cyclin D1 (Thr286)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85246-1-PBS targets Phospho-Cyclin D1 (Thr286) in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin D1
别名 Cyclin D1 (Thr286), p Cyclin D1, p Cyclin D1 (Thr286), p-Cyclin D1, p-Cyclin D1 (Thr286)
计算分子量 295 aa, 34 kDa
观测分子量34 kDa
GenBank蛋白编号BC000076
基因名称 Cyclin D1
Gene ID (NCBI) 595
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP24385
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclin D1 is an important regulator of G1-S phase cell cycle transition. Active cyclin D1-cyclin dependent kinase 4/6 complexes phosphorylate retinoblastoma protein, resulting in release of sequestered E2F transcription factors and subsequent expression of genes required for progression into S phase. Cyclin D1 was localized to the nucleus and its rapid ubiquitin-dependent degradation shown to require phosphorylation at Thr286 by glycogen synthase kinase 3β (GSK3β). (PMID: 16504004)

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