验证数据展示
经过测试的应用
| Positive WB detected in | Calyculin A treated HEK-293 cells |
| Positive IHC detected in | human prostate cancer tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive ChIP-qPCR detected in | Forskolin (30 μM, 1 h) HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
81871-1-RR targets Phospho-CREB1 (Ser133) in WB, IHC, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA, ChIP-qPCR Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | cAMP responsive element binding protein 1 |
| 别名 | CREB1 (Ser133), p CREB1, p CREB1 (Ser133), p CREB1 Ser133, p-CREB1 |
| 计算分子量 | 341 aa, 35 kDa |
| 观测分子量 | 35-46 kDa |
| GenBank蛋白编号 | BC010636 |
| 基因名称 | CREB1 |
| Gene ID (NCBI) | 1385 |
| RRID | AB_2935430 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P16220 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), mitogen-activated protein kinases (MAPK), and other kinases. The activity of CREB1 in neurons has been correlated with various intracellular processes, including proliferation, differentiation, survival, long-term synaptic potentiation, neurogenesis, and neuronal plasticity. CREB1 belongs to the CREB/activating transcription factor (ATF) family of transcription factors. The antibody also detects the phosphorylated form of the CREB-related protein ATF1. (PMID: 30214393, PMID: 33917483, PMID: 32605164)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Phospho-CREB1 (Ser133) antibody 81871-1-RR | Download protocol |
| WB protocol for Phospho-CREB1 (Ser133) antibody 81871-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
