验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy BCR Ready-To-Use IHC Kit. BCR primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87327-1-RR targets Phospho-BCR (Tyr177) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | breakpoint cluster region |
| 别名 | BCR (Tyr177), p-BCR (Tyr177), p-BCR (Y177), p-BCR Tyr177, Phospho BCR (Tyr177) |
| 计算分子量 | 143 kDa |
| 观测分子量 | 210 kDa |
| GenBank蛋白编号 | NM_004327 |
| 基因名称 | BCR |
| Gene ID (NCBI) | 613 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P11274 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The aminoterminal BCR-encoded sequences of BCR-ABL contain a tyrosine-phosphorylated site that binds the SH2 domain of the adaptor protein GRB2. It is now evident that the phosphorylation of BCR Tyr177 is essential for BCR-ABL-mediated leukemogenesis, and its mutation largely abolishes GRB2 binding and diminishes BCR-ABL-induced Ras activation. (PMID: 22156549)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-BCR (Tyr177) antibody 87327-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
