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验证数据展示

  • WB analysis of HEK-293 using 86330-1-RR

    WB analysis of HEK-293 using 86330-1-RR

    Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 86330-1-RR (Phospho-ATR (Ser428) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

  • IF Staining of HEK-293 using 86330-1-RR

    IF Staining of HEK-293 using 86330-1-RR

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HEK-293 cells using Phospho-ATR (Ser428) antibody (86330-1-RR, Clone: 250988D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Phospho-ATR (Ser428) Recombinant monoclonal antibody

Phospho-ATR (Ser428) Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86330-1-RR

产品说明书

CloneNo. 250988D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

ATR (Ser428), p ATR (Ser428), p-ATR, p-ATR (Ser428), p-ATR-Ser428

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy ATR Ready-To-Use IHC Kit. ATR primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86330-1-RR targets Phospho-ATR (Ser428) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ataxia telangiectasia and Rad3 related
别名 ATR (Ser428), p ATR (Ser428), p-ATR, p-ATR (Ser428), p-ATR-Ser428
计算分子量 301 kDa
观测分子量300 kDa
GenBank蛋白编号NM_001184
基因名称 ATR
Gene ID (NCBI) 545
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13535
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ataxia-telangiectasia and Rad3 related (ATR) is a serine/threonine kinase and a member of the phosphatidylinositol 3-kinase-related kinase (PIKK) family, particularly the ataxia telangiectasia mutated (ATM) subfamily. The phosphorylation site of ATR is located at Ser428 and is crucial for proper ATR function. In a series of breast cancer studies, high ATR expression and activation were significantly associated with higher tumor stage, mitotic index, pleomorphism, lymphovascular invasion, and poor survival. (PMID: 33854565)

实验方案

Product Specific Protocols
WB protocol for Phospho-ATR (Ser428) antibody 86330-1-RRDownload protocol
IF protocol for Phospho-ATR (Ser428) antibody 86330-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols