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验证数据展示

  • WB analysis of HEK-293 using 86330-1-RR (same clone as 86330-1-PBS)

    WB analysis of HEK-293 using 86330-1-RR (same clone as 86330-1-PBS)

    Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 86330-1-RR (Phospho-ATR (Ser428) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 86330-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 86330-1-RR (same clone as 86330-1-PBS)

    IF Staining of HEK-293 using 86330-1-RR (same clone as 86330-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HEK-293 cells using Phospho-ATR (Ser428) antibody (86330-1-RR, Clone: 250988D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86330-1-PBS in a different storage buffer formulation.

Phospho-ATR (Ser428) Recombinant monoclonal antibody, PBS Only

Phospho-ATR (Ser428) Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86330-1-PBS

产品说明书

CloneNo. 250988D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

ATR (Ser428), p ATR (Ser428), p-ATR, p-ATR (Ser428), p-ATR-Ser428

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ATR Ready-To-Use IHC Kit. ATR primary antibody included.

产品信息

86330-1-PBS targets Phospho-ATR (Ser428) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ataxia telangiectasia and Rad3 related
别名 ATR (Ser428), p ATR (Ser428), p-ATR, p-ATR (Ser428), p-ATR-Ser428
计算分子量 301 kDa
观测分子量300 kDa
GenBank蛋白编号NM_001184
基因名称 ATR
Gene ID (NCBI) 545
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13535
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ataxia-telangiectasia and Rad3 related (ATR) is a serine/threonine kinase and a member of the phosphatidylinositol 3-kinase-related kinase (PIKK) family, particularly the ataxia telangiectasia mutated (ATM) subfamily. The phosphorylation site of ATR is located at Ser428 and is crucial for proper ATR function. In a series of breast cancer studies, high ATR expression and activation were significantly associated with higher tumor stage, mitotic index, pleomorphism, lymphovascular invasion, and poor survival. (PMID: 33854565)

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