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验证数据展示

  • WB analysis using 80072-2-RR (same clone as 80072-2-PBS)

    WB analysis using 80072-2-RR (same clone as 80072-2-PBS)

    Non-treated, phosphatase inhibitor treated and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80072-2-RR (Phospho-ASK1 (Ser83) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a control. This data was developed using the same antibody clone with 80072-2-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 80072-2-RR (same clone as 80072-2-PBS)

    FC experiment of HEK-293 using 80072-2-RR (same clone as 80072-2-PBS)

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-ASK1 (Ser83) Recombinant antibody (80072-2-RR, Clone:241532B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80072-2-PBS in a different storage buffer formulation.

Phospho-ASK1 (Ser83) Recombinant antibody, PBS Only

Phospho-ASK1 (Ser83) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 80072-2-PBS

产品说明书

CloneNo. 241532B3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

P ASK1 (Ser83), P-ASK1 (Ser83), Phos ASK1 (Ser83), Phos-ASK1 (Ser83), ASK1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

80072-2-PBS targets Phospho-ASK1 (Ser83) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitogen-activated protein kinase kinase kinase 5
别名 P ASK1 (Ser83), P-ASK1 (Ser83), Phos ASK1 (Ser83), Phos-ASK1 (Ser83), ASK1
计算分子量 155 kDa
观测分子量150 kDa
GenBank蛋白编号BC054503
基因名称 ASK1
Gene ID (NCBI) 4217
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ99683
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. ASK1 is reportedly regulated by phosphorylation and dephosphorylation at four different phosphorylation sites. ASK1 activation is accompanied by phosphorylation of Thr845 and dephosphorylation of Ser83, Ser967, and Ser1034. STRAP actually decreased Thr838 (corresponding to Thr845 in mice) phosphorylation but increased Ser83 and Ser967 phosphorylation, an effect that would inactivate ASK1. (PMID: 19880523)