• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 80209-6-RR (same clone as 80209-6-PBS)

    WB analysis using 80209-6-RR (same clone as 80209-6-PBS)

    Non-treated C2C12 cells and H2O2 treated C2C12 cells were subjected to SDS PAGE followed by western blot with 80209-6-RR (Phospho-AMPK Alpha (Thr172) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80209-6-PBS in a different storage buffer formulation.

  • WB analysis using 80209-6-RR (same clone as 80209-6-PBS)

    WB analysis using 80209-6-RR (same clone as 80209-6-PBS)

    Non-treated, phosphatase inhibitor treated and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80209-6-RR (Phospho-AMPK Alpha (Thr172) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80209-6-PBS in a different storage buffer formulation.

  • Schematic diagram of the phosphorylation sites of AMPK Alpha.

    Schematic diagram of the phosphorylation sites of AMPK Alpha.

    Although protein-specific nomenclature varies, the modification site is conserved.

  • FC experiment of HEK-293 using 80209-6-RR (same clone as 80209-6-PBS)

    FC experiment of HEK-293 using 80209-6-RR (same clone as 80209-6-PBS)

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-AMPK Alpha (Thr172) Recombinant monoclonal antibody (80209-6-RR, Clone:242923D4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 90% MeOH . This data was developed using the same antibody clone with 80209-6-PBS in a different storage buffer formulation.

Phospho-AMPK Alpha (Thr172) Recombinant monoclonal antibody, PBS Only

Phospho-AMPK Alpha (Thr172) Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 80209-6-PBS

产品说明书

CloneNo. 242923D4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Indirect ELISA

别名

P-AMPK Alpha 2 (Thr172), Phos AMPK Alpha, Phos-AMPK Alpha 1 (Thr183), Phos-AMPK Alpha 2 (Thr172), Phospho-AMPK Alpha 1 (Thr183)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy PRKAA1 Ready-To-Use IHC Kit. PRKAA1 primary antibody included.

产品信息

80209-6-PBS targets Phospho-AMPK Alpha (Thr172) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein kinase, AMP-activated, alpha 1 catalytic subunit
别名 P-AMPK Alpha 2 (Thr172), Phos AMPK Alpha, Phos-AMPK Alpha 1 (Thr183), Phos-AMPK Alpha 2 (Thr172), Phospho-AMPK Alpha 1 (Thr183)
观测分子量 64 kDa
GenBank蛋白编号BC048980
基因名称 AMPK Alpha 1
Gene ID (NCBI) 5562
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13131
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AMPK is a serine/threonine protein kinase complex consisting of a catalytic α-subunit (α1 and α2), a scaffolding β-subunit (β1 and β2), and a regulatory γ-subunit (γ1, γ2, and γ3). Ubiquitous expression of AMPKα1-, β1-, and γ1-subunits in many tissues makes the α1β1γ1 complex a reference for AMPK assays to identify AMPK activators. AMPK is generally quiescent under normal conditions but is activated in response to signals and stresses that increase the AMP/ATP ratio, such as hypoglycemia, strenuous exercise, anoxia, and ischemia. An increase in the ratio of AMP/ATP activates AMPK by several mechanisms, including direct allosteric activation and covalent modification in which an AMP-dependent AMPK kinase (AMPKK) phosphorylates the α subunit on Thr172. Once activated, AMPK switches on catabolic pathways that generate ATP, while switching off ATP-consuming processes (e.g., biosynthesis, cell growth, and proliferation), and in doing so acts as an "energy gauge". (PMID: 27034026, PMID: 21980456, PMID: 27600021)
This antibody can recognize the phosphorylation sites of Thr183 in AMPK Alpha 1 and Thr172 in AMPK Alpha 2.

||
New chat

Able™

正在加载，请稍候...