验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, λ phosphatase treated MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87220-1-RR targets Phospho-AKT (Thr450) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | v-akt murine thymoma viral oncogene homolog 1 |
| 别名 | AKT (phospho T450), AKT1 (phospho T450), p-AKT, p-AKT (Thr450), p-AKT T450 |
| 计算分子量 | 56 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | NM_005163 |
| 基因名称 | AKT1 |
| Gene ID (NCBI) | 207 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P31749 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Phospho-AKT (Thr450) is recognized as the phosphorylation site on AKT (protein kinase B) at the threonine 450 residue. This site is referred to as the turn phosphorylation site and was previously thought to be a result of autophosphorylation, but more recent work has shown that its phosphorylation is controlled by TORC2 activity. This phosphorylation site is important in other AGC kinases, but the role in AKT signaling had not been determined. (PMID: 21670491)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-AKT (Thr450) antibody 87220-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
