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验证数据展示

  • WB analysis using 87220-1-RR (same clone as 87220-1-PBS)

    WB analysis using 87220-1-RR (same clone as 87220-1-PBS)

    Non-treated MCF-7 cells, phosphatase inhibitor treated MCF-7 cells and λ phosphatase treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 87220-1-RR (Phospho-AKT (Thr450) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87220-1-PBS in a different storage buffer formulation.

Phospho-AKT (Thr450) Recombinant monoclonal antibody, PBS Only

Phospho-AKT (Thr450) Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 87220-1-PBS

产品说明书

CloneNo. 252456B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

AKT (phospho T450), AKT1 (phospho T450), p-AKT, p-AKT (Thr450), p-AKT T450

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy AKT Ready-To-Use IHC Kit. AKT primary antibody included.

产品信息

87220-1-PBS targets Phospho-AKT (Thr450) in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-akt murine thymoma viral oncogene homolog 1
别名 AKT (phospho T450), AKT1 (phospho T450), p-AKT, p-AKT (Thr450), p-AKT T450
计算分子量 56 kDa
观测分子量60 kDa
GenBank蛋白编号NM_005163
基因名称 AKT1
Gene ID (NCBI) 207
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP31749
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Phospho-AKT (Thr450) is recognized as the phosphorylation site on AKT (protein kinase B) at the threonine 450 residue. This site is referred to as the turn phosphorylation site and was previously thought to be a result of autophosphorylation, but more recent work has shown that its phosphorylation is controlled by TORC2 activity. This phosphorylation site is important in other AGC kinases, but the role in AKT signaling had not been determined. (PMID: 21670491)

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