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验证数据展示

  • WB analysis using 30437-1-AP (same clone as 30437-1-PBS)

    WB analysis using 30437-1-AP (same clone as 30437-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30437-1-AP (Periphilin 1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30437-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 30437-1-AP (same clone as 30437-1-PBS)

    IHC staining of mouse colon using 30437-1-AP (same clone as 30437-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30437-1-AP (Periphilin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30437-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 30437-1-AP (same clone as 30437-1-PBS)

    IHC staining of mouse colon using 30437-1-AP (same clone as 30437-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30437-1-AP (Periphilin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30437-1-PBS in a different storage buffer formulation.

Periphilin 1 Polyclonal antibody, PBS Only

Periphilin 1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30437-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

PPHLN1, Gastric cancer antigen Ga50, HSPC206, HSPC232, Periphilin-1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30437-1-PBS targets Periphilin 1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32768

Product name: Recombinant human PPHLN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 33-200 aa of BC039832

Sequence: ESGYRWTRDDHSASRQPEYRDMRDGFRRKSFYSSHYARERSPYKRDNTFFRESPVGRKDSPHSRSGSSVSSRSYSPERSKSYSFHQSQHRKSVRPGASYKRQNEGNPERDKERPVQSLKTSRDTSPSSGSAVSSSKVLDKPSRLTEKELAEAASKWAAEKLEKSDESN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 periphilin 1
别名 PPHLN1, Gastric cancer antigen Ga50, HSPC206, HSPC232, Periphilin-1
计算分子量 458 aa, 53 kDa
观测分子量37-70 kDa
GenBank蛋白编号BC039832
基因名称 PPHLN1
Gene ID (NCBI) 51535
RRIDAB_3742343
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NEY8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Periphilin 1 is a component of the HUSH complex, which is a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is likely necessary for maintaining transcriptional silencing by promoting the recruitment of SETDB1, a histone methyltransferase that mediates the deposition of additional H3K9me3. Periphilin 1 contributes to the maintenance of the HUSH complex at chromatin (PMID: 26022416).

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