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验证数据展示

  • WB analysis using 30437-1-AP

    WB analysis using 30437-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30437-1-AP (Periphilin 1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 30437-1-AP

    IHC staining of mouse colon using 30437-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30437-1-AP (Periphilin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 30437-1-AP

    IHC staining of mouse colon using 30437-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30437-1-AP (Periphilin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Periphilin 1 Polyclonal antibody

Periphilin 1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30437-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

PPHLN1, Gastric cancer antigen Ga50, HSPC206, HSPC232, Periphilin-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, Calu-1 cells, Jurkat cells, MG-63 cells, U2OS cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30437-1-AP targets Periphilin 1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32768

Product name: Recombinant human PPHLN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 33-200 aa of BC039832

Sequence: ESGYRWTRDDHSASRQPEYRDMRDGFRRKSFYSSHYARERSPYKRDNTFFRESPVGRKDSPHSRSGSSVSSRSYSPERSKSYSFHQSQHRKSVRPGASYKRQNEGNPERDKERPVQSLKTSRDTSPSSGSAVSSSKVLDKPSRLTEKELAEAASKWAAEKLEKSDESN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 periphilin 1
别名 PPHLN1, Gastric cancer antigen Ga50, HSPC206, HSPC232, Periphilin-1
计算分子量 458 aa, 53 kDa
观测分子量37-70 kDa
GenBank蛋白编号BC039832
基因名称 PPHLN1
Gene ID (NCBI) 51535
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NEY8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Periphilin 1 is a component of the HUSH complex, which is a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is likely necessary for maintaining transcriptional silencing by promoting the recruitment of SETDB1, a histone methyltransferase that mediates the deposition of additional H3K9me3. Periphilin 1 contributes to the maintenance of the HUSH complex at chromatin (PMID: 26022416).

实验方案

Product Specific Protocols
IHC protocol for Periphilin 1 antibody 30437-1-APDownload protocol
WB protocol for Periphilin 1 antibody 30437-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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