验证数据展示
经过测试的应用
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66317 targets Peripherin in IF/ICC applications and shows reactivity with human, rat, pig samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, rat, pig |
| 免疫原 |
CatNo: Ag11116 Product name: Recombinant human Peripherin protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 155-470 aa of BC032703 Sequence: LELLGRERDRVQVERDGLAEDLAALKQRLEEETRKREDAEHNLVLFRKDVDDATLSRLELERKIESLMDEIEFLKKLHEEELRDLQVSVESQQVQQVEVEATVKPELTAALRDIRAQYESIAAKNLQEAEEWYKSKYADLSDAANRNHEALRQAKQEMNESRRQIQSLTCEVDGLRGTNEALLRQLRELEEQFALEAGGYQAGAARLEEELRQLKEEMARHLREYQELLNVKMALDIEIATYRKLLEGEESRISVPVHSFASLNIKTTVPEVEPPQDSHSRKTVLIKTIETRNGEVVTESQKEQRSELDKSSAHSY 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | peripherin |
| 别名 | NEF4, Neurofilament 4, peripherin, PRPH, PRPH1 |
| 计算分子量 | 470 aa, 54 kDa |
| 观测分子量 | 50-54 kDa |
| GenBank蛋白编号 | BC032703 |
| 基因名称 | Peripherin |
| Gene ID (NCBI) | 5630 |
| RRID | AB_3084232 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 激发激光 | Blue laser (488 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P41219 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Peripherin is a neuron-specific intermediate filament protein that is particularly expressed in peripheral nervous system. It is used to study peripheral nerve development and regeneration, as it is highly expressed during elongation of axon. Peripherin is a routine marker for unmyelinated neurons in trigeminal ganglion (TG) and the dorsal root ganglion (DRG). Peripherin is also expressed in peri-insular areas of islets and it has been identified as a key autoantigen for type 1A diabetes.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Peripherin antibody CL488-66317 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
