验证数据展示
经过测试的应用
| Positive WB detected in | RAW 264.7 cells |
| Positive IHC detected in | human breast cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
| IHC | See 1 publications below |
产品信息
22172-1-AP targets Paxillin in WB, IF, IHC, ELISA applications and shows reactivity with human, Canine, mouse samples.
| 经测试应用 | WB, IF, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, Canine, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag17702 Product name: Recombinant human PXN protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-267 aa of BC136787 Sequence: MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQPSGSRFIHQQPQSSSPVYGSSAKTSSVSNPQDSVGSPCSRVGEEEHVYSFPNKQKSAEPSPTVMSTSLGSNLSELDRLLLELNAVQHNPPGFPADEANSSPPLPGALSPLYGVPETNSPLGGKAGPLTKEKPKRNGGRGLEDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSTQQQTRISASSATRELD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | paxillin |
| 别名 | paxillin, PXN |
| 计算分子量 | 591 aa, 65 kDa |
| GenBank蛋白编号 | BC136787 |
| 基因名称 | Paxillin |
| Gene ID (NCBI) | 5829 |
| RRID | AB_2879016 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49023 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Paxillin antibody 22172-1-AP | Download protocol |
| IHC protocol for Paxillin antibody 22172-1-AP | Download protocol |
| WB protocol for Paxillin antibody 22172-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer. | ||
Sci Rep A novel cystathionine γ-lyase inhibitor, I194496, inhibits the growth and metastasis of human TNBC via downregulating multiple signaling pathways. | ||
Sci Rep Interferon-inducible protein, IFIX, has tumor-suppressive effects in oral squamous cell carcinoma | ||
Int J Oncol Cystathionine‑γ‑lyase promotes the metastasis of breast cancer via the VEGF signaling pathway. | ||
Cell Death Discov A novel chemical inhibitor suppresses breast cancer cell growth and metastasis through inhibiting HPIP oncoprotein. | ||
Cell Signal Activated CRH receptors inhibit autophagy by repressing conversion of LC3BI to LC3BII. |
