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验证数据展示

  • IF Staining of mouse cerebellum tissue using 29312-1-AP (same clone as 29312-1-PBS)

    IF Staining of mouse cerebellum tissue using 29312-1-AP (same clone as 29312-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed adult mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:1500 and Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L). (Image produced by Watt Lab at McGill University) This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

  • WB analysis using 29312-1-AP (same clone as 29312-1-PBS)

    WB analysis using 29312-1-AP (same clone as 29312-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

    IHC staining of mouse cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

    IF Staining of mouse cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

    IHC staining of mouse cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

    IF Staining of mouse cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

Parvalbumin Polyclonal antibody, PBS Only

Parvalbumin Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 29312-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

PVALB, Parvalbumin alpha

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29312-1-PBS targets Parvalbumin in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30189

Product name: Recombinant human PVALB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-110 aa of NM_002854

Sequence: SMTDLLNAEDIKKAVGAFSATDSFDHKKFFQMVGLKKKSADDVKKVFHMLDKDKSGFIEEDELGFILKGFSPDARDLSAKETKMLMAAGDKDGDGKIGVDEFSTLVAES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 parvalbumin
别名 PVALB, Parvalbumin alpha
计算分子量 12 kDa
观测分子量15 kDa
GenBank蛋白编号NM_002854
基因名称 Parvalbumin
Gene ID (NCBI) 5816
RRIDAB_2918280
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20472
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PVALB is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. PVALB is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. It is thought to be involved in muscle relaxation. Monomeric (12 kDa) and dimeric (24 kDa) forms, as well as trimeric Parvalbumin (38 kDa), have been described in a literature (PMID: 19616851).

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