验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse cerebellum tissue, mouse brain tissue, rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse cerebellum tissue, mouse brain tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29312-1-AP targets Parvalbumin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, rat |
| 免疫原 | Parvalbumin fusion protein Ag30189 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | parvalbumin |
| 别名 | PVALB, Parvalbumin alpha |
| 计算分子量 | 12 kDa |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | NM_002854 |
| 基因名称 | Parvalbumin |
| Gene ID (NCBI) | 5816 |
| RRID | AB_2918280 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P20472 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PVALB is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. PVALB is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. It is thought to be involved in muscle relaxation. Monomeric (12 kDa) and dimeric (24 kDa) forms, as well as trimeric Parvalbumin (38 kDa), have been described in a literature (PMID: 19616851).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Parvalbumin antibody 29312-1-AP | Download protocol |
| IHC protocol for Parvalbumin antibody 29312-1-AP | Download protocol |
| IF protocol for Parvalbumin antibody 29312-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Mol Neurosci PGC-1α regulates critical period onset/closure, mediating cortical plasticity | ||
Physiol Res High-Intensity Interval Training Increases Osteoarthritis-Associated Pain-Sensitive Threshold Through Reduction of Perineuronal Nets of the Medial Prefrontal Cortex in Rats | ||
Transl Androl Urol Whole body vibration training promotes proprioceptive pathway for the treatment of stress urinary incontinence in rats | ||
Mol Neurobiol Oxidative Stress-mediated Loss of Hippocampal Parvalbumin Interneurons Contributes to Memory Precision Decline After Acute Sleep Deprivation | ||
Cell Rep Methods A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution | ||
STAR Protoc Protocol for fine casting, imaging, and analysis of murine vascular networks with VALID |
