验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse skeletal muscle tissue, rat brain tissue, rat skeletal muscle tissue |
| Positive IHC detected in | mouse cerebellum tissue, mouse brain tissue, rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse cerebellum tissue, mouse brain tissue, rat cerebellum tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29312-1-AP targets Parvalbumin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, rat |
| 免疫原 |
CatNo: Ag30189 Product name: Recombinant human PVALB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-110 aa of NM_002854 Sequence: SMTDLLNAEDIKKAVGAFSATDSFDHKKFFQMVGLKKKSADDVKKVFHMLDKDKSGFIEEDELGFILKGFSPDARDLSAKETKMLMAAGDKDGDGKIGVDEFSTLVAES 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | parvalbumin |
| 别名 | PVALB, Parvalbumin alpha |
| 计算分子量 | 12 kDa |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | NM_002854 |
| 基因名称 | Parvalbumin |
| Gene ID (NCBI) | 5816 |
| RRID | AB_2918280 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P20472 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PVALB is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. PVALB is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. It is thought to be involved in muscle relaxation. Monomeric (12 kDa) and dimeric (24 kDa) forms, as well as trimeric Parvalbumin (38 kDa), have been described in a literature (PMID: 19616851).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Parvalbumin antibody 29312-1-AP | Download protocol |
| IHC protocol for Parvalbumin antibody 29312-1-AP | Download protocol |
| WB protocol for Parvalbumin antibody 29312-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun A distinct PP2A subunit regulates local protein phosphorylation at the axon initial segment. | ||
Nat Commun An attention-demanding hunting paradigm engages the superior colliculus-zona incerta circuit mediating analgesia in male mice. | ||
Acta Pharm Sin B The Kv4.3 channel on c-low threshold mechanoreceptors in DRG is a key mediator transforming tactile stimuli into nociceptive signals in chemotherapy-induced neuropathy. | ||
Br J Pharmacol In rats, neurodevelopmental disorders induced by maternal hypoxia are associated with attenuated excitatory neurotransmission in the cingulate cortex. | ||
Transl Psychiatry Prenatal valproic acid exposure alters striatal proteomic signatures associated with autism spectrum disorder in mice. | ||
Cell Rep Methods A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution |
