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验证数据展示

  • WB analysis using 86398-1-RR

    WB analysis using 86398-1-RR

    Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 86398-1-RR (Phospho-Tyrosine antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

  • IF Staining of A431 using 86398-1-RR

    IF Staining of A431 using 86398-1-RR

    Immunofluorescent analysis of (4% PFA) fixed hEGF treated A431 cells using Phospho-Tyrosine antibody (86398-1-RR, Clone: 250495G7 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of A431 using 86398-1-RR

    IF Staining of A431 using 86398-1-RR

    Immunofluorescent analysis of (4% PFA) fixed hEGF treated A431 cells using Phospho-Tyrosine antibody (86398-1-RR, Clone: 250495G7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Phospho-Tyrosine Recombinant monoclonal antibody

Phospho-Tyrosine Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86398-1-RR

产品说明书

CloneNo. 250495G7

宿主/亚型

Rabbit / IgG

种属反应性

-

应用

WB, IF/ICC, ELISA

别名

P Tyr, p Tyrosine, pan phospho, pan-phospho, Pan-phospho-Tyr

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inEGF treated A431 cells
Positive IF/ICC detected inhEGF treated A431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86398-1-RR targets Phospho-Tyrosine in WB, IF/ICC, ELISA applications and shows reactivity with - samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 -
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Phospho-Tyrosine
别名 P Tyr, p Tyrosine, pan phospho, pan-phospho, Pan-phospho-Tyr
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phospho-Tyrosine antibody refers to a class of pan-specific antibodies or detection reagents that recognize all phosphorylated tyrosine residues regardless of the surrounding amino-acid sequence. These tools are widely used to monitor global tyrosine phosphorylation events in signal-transduction studies, cancer research and drug-discovery screens.
This antibody does not cross-react with peptides containing Phospho-Serine or Phospho-Threonine.

实验方案

Product Specific Protocols
IF protocol for Phospho-Tyrosine antibody 86398-1-RRDownload protocol
WB protocol for Phospho-Tyrosine antibody 86398-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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