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验证数据展示

  • WB analysis using 21845-1-AP (same clone as 21845-1-PBS)

    WB analysis using 21845-1-AP (same clone as 21845-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21845-1-AP (PYROXD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21845-1-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 21845-1-AP (same clone as 21845-1-PBS)

    IHC staining of rat liver using 21845-1-AP (same clone as 21845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 21845-1-AP (PYROXD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21845-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 21845-1-AP (same clone as 21845-1-PBS)

    IHC staining of mouse liver using 21845-1-AP (same clone as 21845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21845-1-AP (PYROXD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21845-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 21845-1-AP (same clone as 21845-1-PBS)

    IF Staining of U2OS using 21845-1-AP (same clone as 21845-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PYROXD2 antibody (21845-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 21845-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 21845-1-AP (same clone as 21845-1-PBS)

    IF Staining of U2OS using 21845-1-AP (same clone as 21845-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PYROXD2 antibody (21845-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21845-1-PBS in a different storage buffer formulation.

PYROXD2 Polyclonal antibody, PBS Only

PYROXD2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21845-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

C10orf33, FP3420, YueF

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

21845-1-PBS targets PYROXD2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15004

Product name: Recombinant human C10orf33 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 232-581 aa of BC006131

Sequence: DQWFESEPLKATLATDAVIGAMTSPHTPGSGYVLLHHVMGGLEGMQGAWGYVQGGMGALSDAIASSATTHGASIFTEKTVAKVQVNSEGCVQGVVLEDGTEVRSKMVLSNTSPQITFLKLTPQEWLPEEFLERISQLDTRSPVTKINVAVDRLPSFLAAPNAPRGQPLPHHQCSIHLNCEDTLLLHQAFEDAMDGLSSHRPVIELCIPSSLDPTLAPPGCHVVSLFTQYTPYTLAGGKAWDEQERDAYADRVFDCIEVYAPGFKDSVVGRDILTPPDLERIFGLPGGNIFHCAMSLDQLYFARPVPLHSGYRCPLQGLYLCGSGAHPGGGVMGAAGRNAAHVAFRDLKSM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 10 open reading frame 33
别名 C10orf33, FP3420, YueF
计算分子量 581 aa, 63 kDa
观测分子量63 kDa
GenBank蛋白编号BC006131
基因名称 PYROXD2
Gene ID (NCBI) 84795
RRIDAB_3669377
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N2H3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Pyridine Nucleotide-Disulfide Oxidoreductase Domain 2 (PYROXD2, previously called YueF, C10orf33) is a mitochondrial inner membrane/matrix-residing protein and is reported to regulate mitochondrial function. PYROXD2 is highly expressed in the cytoplasm of normal cells and tissues but is expressed at lower levels in corresponding cancer cells, including liver, lung and renal cell carcinoma and bladder cancer cells (PMID: 29048625, PMID: 35606887).

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