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验证数据展示

  • WB analysis using 21845-1-AP

    WB analysis using 21845-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21845-1-AP (PYROXD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat liver using 21845-1-AP

    IHC staining of rat liver using 21845-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 21845-1-AP (PYROXD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 21845-1-AP

    IHC staining of mouse liver using 21845-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21845-1-AP (PYROXD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PYROXD2 Polyclonal antibody

PYROXD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21845-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

C10orf33, FP3420, YueF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, HEK-293T cells, L02 cells
Positive IHC detected inmouse liver tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21845-1-AP targets PYROXD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PYROXD2 fusion protein Ag15004 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 10 open reading frame 33
别名 C10orf33, FP3420, YueF
计算分子量 581 aa, 63 kDa
观测分子量63 kDa
GenBank蛋白编号BC006131
基因名称 PYROXD2
Gene ID (NCBI) 84795
RRIDAB_3669377
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N2H3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pyridine Nucleotide-Disulfide Oxidoreductase Domain 2 (PYROXD2, previously called YueF, C10orf33) is a mitochondrial inner membrane/matrix-residing protein and is reported to regulate mitochondrial function. PYROXD2 is highly expressed in the cytoplasm of normal cells and tissues but is expressed at lower levels in corresponding cancer cells, including liver, lung and renal cell carcinoma and bladder cancer cells (PMID: 29048625, PMID: 35606887).

实验方案

Product Specific Protocols
WB protocol for PYROXD2 antibody 21845-1-APDownload protocol
IHC protocol for PYROXD2 antibody 21845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols