• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HSC-T6 using 66769-1-Ig (same clone as 66769-1-PBS)

    WB analysis of HSC-T6 using 66769-1-Ig (same clone as 66769-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66769-1-Ig (PYGL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66769-1-PBS in a different storage buffer formulation.

  • WB analysis using 66769-1-Ig (same clone as 66769-1-PBS)

    WB analysis using 66769-1-Ig (same clone as 66769-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66769-1-Ig (PYGL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66769-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66769-1-Ig (same clone as 66769-1-PBS)

    WB analysis of HeLa using 66769-1-Ig (same clone as 66769-1-PBS)

    WB result of PYGL antibody (66769-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGL transfected HeLa cells. This data was developed using the same antibody clone with 66769-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 66769-1-Ig (same clone as 66769-1-PBS)

    IF Staining of U-251 using 66769-1-Ig (same clone as 66769-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using PYGL antibody (66769-1-Ig, Clone: 1G12D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66769-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PYGL Monoclonal antibody, PBS Only

PYGL Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66769-1-PBS

产品说明书

CloneNo. 1G12D8

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IF/ICC, Indirect ELISA

别名

Glycogen phosphorylase, liver form, EC:2.4.1.1, 1G12D8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

66769-1-PBS targets PYGL in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 PYGL fusion protein Ag8550 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphorylase, glycogen, liver
别名 Glycogen phosphorylase, liver form, EC:2.4.1.1, 1G12D8
计算分子量 846 aa, 97 kDa
观测分子量 97 kDa
GenBank蛋白编号BC009895
基因名称 PYGL
Gene ID (NCBI) 5836
RRIDAB_2882115
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP06737
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glycogen phosphorylase L (PYGL) is one of the gene related to hypoxia metabolism and was found to be up-regulated in head and neck squamous cell carcinomas (HNSCCs) and breast cancers. Glycogen synthases (GSs) and glycogen phosphorylases (GPs) catalyze the key steps of glycogen synthesis and breakdown, while glycogen phosphorylase has three isoforms: PYGM (muscle), PYGL (liver), and PYGB (brain) (PMID: 37063425, 9529348). High PYGL expression plays an independent role in predicting the poor prognosis of glioma patients (PMID: 34177761).