验证数据展示
经过测试的应用
| Positive WB detected in | HSC-T6 cells, HeLa cells, HepG2 cells, L02 cells, SMMC-7721 cells |
| Positive IF/ICC detected in | U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
66769-1-Ig targets PYGL in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human |
| 免疫原 | PYGL fusion protein Ag8550 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | phosphorylase, glycogen, liver |
| 别名 | Glycogen phosphorylase, liver form, EC:2.4.1.1, 1G12D8 |
| 计算分子量 | 846 aa, 97 kDa |
| 观测分子量 | 97 kDa |
| GenBank蛋白编号 | BC009895 |
| 基因名称 | PYGL |
| Gene ID (NCBI) | 5836 |
| RRID | AB_2882115 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P06737 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glycogen phosphorylase L (PYGL) is one of the gene related to hypoxia metabolism and was found to be up-regulated in head and neck squamous cell carcinomas (HNSCCs) and breast cancers. Glycogen synthases (GSs) and glycogen phosphorylases (GPs) catalyze the key steps of glycogen synthesis and breakdown, while glycogen phosphorylase has three isoforms: PYGM (muscle), PYGL (liver), and PYGB (brain) (PMID: 37063425, 9529348). High PYGL expression plays an independent role in predicting the poor prognosis of glioma patients (PMID: 34177761).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PYGL antibody 66769-1-Ig | Download protocol |
| IF protocol for PYGL antibody 66769-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |