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验证数据展示

  • WB analysis using 11512-1-AP (same clone as 11512-1-PBS)

    WB analysis using 11512-1-AP (same clone as 11512-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11512-1-AP (PUS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 11512-1-AP (same clone as 11512-1-PBS)

    IHC staining of human ovary tumor using 11512-1-AP (same clone as 11512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11512-1-AP (PUS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PUS1 antibody included
  • IHC staining of human ovary tumor using 11512-1-AP (same clone as 11512-1-PBS)

    IHC staining of human ovary tumor using 11512-1-AP (same clone as 11512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11512-1-AP (PUS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PUS1 antibody included
  • IF Staining of MCF-7 using 11512-1-AP (same clone as 11512-1-PBS)

    IF Staining of MCF-7 using 11512-1-AP (same clone as 11512-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PUS1 antibody (11512-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PUS1 Polyclonal antibody, PBS Only

PUS1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11512-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EC:5.4.99.-, EC:5.4.99.12, MLASA, PP8985, pseudouridylate synthase 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PUS1 Ready-To-Use IHC Kit. PUS1 primary antibody included.

产品信息

11512-1-PBS targets PUS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2071

Product name: Recombinant human PUS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 128-427 aa of BC019320

Sequence: ENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFAHKDRDVQDETYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTEKVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNFSATALTAGGTGAKVPSPLEGSEGDGDTD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pseudouridylate synthase 1
别名 EC:5.4.99.-, EC:5.4.99.12, MLASA, PP8985, pseudouridylate synthase 1
计算分子量 427 aa, 47 kDa
观测分子量 40 kDa, 44 kDa
GenBank蛋白编号BC019320
基因名称 PUS1
Gene ID (NCBI) 80324
RRIDAB_2284652
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y606
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PUS1 is a protein-coding gene located on human chromosome 12q24.33, encoding a pseudouridine synthase that catalyzes the isomerization of uridine to pseudouridine (Ψ) in RNA molecules. This post-transcriptional modification is the most abundant RNA modification in cells and plays essential roles in RNA structure stabilization and function. (PMID: 38450158)

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