验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue, HEK-293 cells, HeLa cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy PUS1 Ready-To-Use IHC Kit. PUS1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
11512-1-AP targets PUS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | PUS1 fusion protein Ag2071 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pseudouridylate synthase 1 |
| 别名 | EC:5.4.99.-, EC:5.4.99.12, MLASA, PP8985, pseudouridylate synthase 1 |
| 计算分子量 | 427 aa, 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC019320 |
| 基因名称 | PUS1 |
| Gene ID (NCBI) | 80324 |
| RRID | AB_2284652 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y606 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PUS1 antibody 11512-1-AP | Download protocol |
| IHC protocol for PUS1 antibody 11512-1-AP | Download protocol |
| IF protocol for PUS1 antibody 11512-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Methods Absolute quantitative and base-resolution sequencing reveals comprehensive landscape of pseudouridine across the human transcriptome | ||
RNA Mapping of pseudouridine residues on viral and cellular transcripts using a novel antibody-based technique. | ||
Pharmgenomics Pers Med PUS1 May Be a Potential Prognostic Biomarker and Therapeutic Target for Hepatocellular Carcinoma
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