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验证数据展示

  • WB analysis using 61022-1-Ig (same clone as 61022-1-PBS)

    WB analysis using 61022-1-Ig (same clone as 61022-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 61022-1-Ig (PURB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 61022-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 61022-1-Ig (same clone as 61022-1-PBS)

    IHC staining of mouse stomach using 61022-1-Ig (same clone as 61022-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 61022-1-Ig (PURB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 61022-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 61022-1-Ig (same clone as 61022-1-PBS)

    IHC staining of mouse stomach using 61022-1-Ig (same clone as 61022-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 61022-1-Ig (PURB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 61022-1-PBS in a different storage buffer formulation.

PURB Monoclonal antibody, PBS Only

PURB Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 61022-1-PBS

产品说明书

CloneNo. 2F5G2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, rabbit

应用

WB, IHC, Indirect ELISA

别名

PURBETA, Purine-rich element-binding protein B, Transcriptional regulator protein Pur-beta

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

61022-1-PBS targets PURB in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原

CatNo: Ag12816

Product name: Recombinant human PURB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-312 aa of BC101735

Sequence: MADGDSGSERGGGGGPCGFQPASRGGGEQETQELASKRLDIQNKRFYLDVKQNAKGRFLKIAEVGAGGSKSRLTLSMAVAAEFRDSLGDFIEHYAQLGPSSPEQLAAGAEEGGGPRRALKSEFLVRENRKYYLDLKENQRGRFLRIRQTVNRGGGGFGAGPGPGGLQSGQTIALPAQGLIEFRDALAKLIDDYGGEDDELAGGPGGGAGGPGGGLYGELPEGTSITVDSKRFFFDVGCNKYGVFLRVSEVKPSYRNAITVPFKAWGKFGGAFCRYADEMKEIQERQRDKLYERRGGGSGGGEESEGEEVDED

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 purine-rich element binding protein B
别名 PURBETA, Purine-rich element-binding protein B, Transcriptional regulator protein Pur-beta
计算分子量 312 aa, 33 kDa
观测分子量 33-40 kDa
GenBank蛋白编号BC101735
基因名称 PURB
Gene ID (NCBI) 5814
RRIDAB_3743258
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96QR8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PURB (Purine-rich element-binding protein B) is a ubiquitously expressed DNA/RNA-binding protein. It localizes to both the nucleus and cytoplasm, reflecting its dual role in transcriptional regulation and RNA metabolism. Its primary function is to bind purine-rich sequences to control gene expression, cell cycle progression, and differentiation. The observed molecular weight of PURB by SDS-PAGE is 33-40 kDa, which is close to its predicted molecular weight of ~33 kDa.

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