验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, pig brain tissue, rat brain tissue, mouse brain tissue, L02 cells, LNCaP cells, human placenta tissue, rat cerebellum tissue, mouse cerebellum tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67305-1-Ig targets PURA in WB, IF, ChIP, RIP, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | IF, ChIP, RIP |
| 经测试反应性 | Human, mouse, rat, pig |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag12164 Product name: Recombinant human PURA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-322 aa of BC036087 Sequence: MADRDSGSEQGGAALGSGGSLGHPGSGSGSGGGGGGGGGGGGSGGGGGGAPGGLQHETQELASKRVDIRNKRFYLDVKQNAKGRFLKIAEVGAGGNKSRLTLSMSVAVEFRDYLGDFIEHYAQLGPSQPPDLAQAQDEPRRALKSEFLVRENRKYYMDLKENQRGRFLRIRQTVNRGPGLGSTQGQTIALPAQGLIEFRDALAKLIDDYGVEEEPAELPEGTSLTVDNKRFFFDVGSNKYGVFMRVSEVKPTYRNSITVPYKVWAKFGHTFCKYSEEMKKIQEKQREKRAACEQLHQQQQQQQEETAAATLLLQGEEEGEED 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | purine-rich element binding protein A |
| 别名 | PUR ALPHA, PUR1, PURA, PURALPHA |
| 计算分子量 | 322 aa, 35 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC036087 |
| 基因名称 | PURA |
| Gene ID (NCBI) | 5813 |
| RRID | AB_2882567 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q00577 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PURA, also named as Transcriptional activator protein Pur-alpha, is a 322 amino acid protein, which belongs to the PUR DNA-binding protein family and can form homodimer or heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1. PURA is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene and may play a role in the initiation of DNA replication and in recombination.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PURA antibody 67305-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
