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Conjugate
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验证数据展示

  • WB analysis using 68533-1-Ig (same clone as 68533-1-PBS)

    WB analysis using 68533-1-Ig (same clone as 68533-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68533-1-Ig (PUF60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68533-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse testis using 68533-1-Ig (same clone as 68533-1-PBS)

    WB analysis of mouse testis using 68533-1-Ig (same clone as 68533-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 68533-1-Ig (PUF60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68533-1-PBS in a different storage buffer formulation.

PUF60 Monoclonal antibody, PBS Only

PUF60 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68533-1-PBS

产品说明书

CloneNo. 1A3A1

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, rabbit

应用

WB, Indirect ELISA

别名

FBP interacting repressor, FIR, FLJ31379, PUF60, Ro binding protein 1, RoBP1, RoBPI, Siah binding protein 1, Siah BP1, SIAHBP1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PUF60 Ready-To-Use IHC Kit. PUF60 primary antibody included.

产品信息

68533-1-PBS targets PUF60 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat, rabbit
免疫原

CatNo: Ag33930

Product name: Recombinant human PUF60 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 243-542 aa of BC008875

Sequence: DPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 poly-U binding splicing factor 60KDa
别名 FBP interacting repressor, FIR, FLJ31379, PUF60, Ro binding protein 1, RoBP1, RoBPI, Siah binding protein 1, Siah BP1, SIAHBP1
计算分子量 60 kDa
观测分子量60 kDa
GenBank蛋白编号BC008875
基因名称 PUF60
Gene ID (NCBI) 22827
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9UHX1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Poly-U-binding factor 60(PUF60) is a splicing factor homologous to and complementary in function to U2AF, which involves in pre-mRNA splicing, a stepwise process initiated by binding. Also PUF60 involves in other nuclear processes such as apoptosis and transcription regulation. It can interact with and repress FUBP1-induced transcriptional activation via the core-TFIIH basal transcription factor. However, it has no repression activity in xeroderma pigmentosum complementation group B (XPB) cells.

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