验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, mouse testis tissue |
Planning an IHC experiment? We recommend our IHCeasy PUF60 Ready-To-Use IHC Kit. PUF60 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
68533-1-Ig targets PUF60 in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, mouse, rat, rabbit |
| 文献引用反应性 | mouse |
| 免疫原 | PUF60 fusion protein Ag33930 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | poly-U binding splicing factor 60KDa |
| 别名 | FBP interacting repressor, FIR, FLJ31379, PUF60, Ro binding protein 1, RoBP1, RoBPI, Siah binding protein 1, Siah BP1, SIAHBP1 |
| 计算分子量 | 60 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC008875 |
| 基因名称 | PUF60 |
| Gene ID (NCBI) | 22827 |
| RRID | AB_3085241 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9UHX1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Poly-U-binding factor 60(PUF60) is a splicing factor homologous to and complementary in function to U2AF, which involves in pre-mRNA splicing, a stepwise process initiated by binding. Also PUF60 involves in other nuclear processes such as apoptosis and transcription regulation. It can interact with and repress FUBP1-induced transcriptional activation via the core-TFIIH basal transcription factor. However, it has no repression activity in xeroderma pigmentosum complementation group B (XPB) cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PUF60 antibody 68533-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |