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验证数据展示

  • IHC staining of mouse brain using 25473-1-AP

    IHC staining of mouse brain using 25473-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25473-1-AP (PTPRT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 25473-1-AP

    IHC staining of mouse brain using 25473-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25473-1-AP (PTPRT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PTPRT Polyclonal antibody

PTPRT Polyclonal Antibody for IHC, ELISA
Cat No. 25473-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

KIAA0283, PTPRT, R PTP T, RPTP rho, RPTPrho

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25473-1-AP targets PTPRT in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, T
别名 KIAA0283, PTPRT, R PTP T, RPTP rho, RPTPrho
计算分子量 164 kDa
GenBank蛋白编号NM_007050
基因名称 PTPRT
Gene ID (NCBI) 11122
RRIDAB_3085797
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14522
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPRT is a member of the type IIB receptor-type protein tyrosine phosphatase family, linked to signal transduction, cell adhesion and neurite extension. PTPRT plays a role in regulation of synaptic formation and neuronal development. It regulates synaptic function and neuronal development through interactions with neuronal adhesion molecules and the dephosphorylation of synaptic molecules. PTPRT is mainly expressed in nervous system (PMID:19816407, PMID: 25748173).

实验方案

Product Specific Protocols
IHC protocol for PTPRT antibody 25473-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols